T71 A Showcase of Undergraduate Research in Hydrogeology (Posters)
Sunday, 25 September 2016
9:00 AM - 5:30 PM
Exhibit Hall E/F
STREAM CONTAMINATION IN THE CITIES OF MILLERSVILLE AND GOODLETTSVILLE, TENNESSEE
Paper number: T71-18
Program: Technical Sessions
Time: 9:00 AM – 5:30 PM
Authors
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Herbert Pat BarronAustin Peay State University, Clarksville, TN
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Kallina DunkleAustin Peay State University, Clarksville, TN
Abstract
In order to locate the source or sources of E. coli stream contamination, water samples have been collected and tested for E. coli since November 2014, with weekly testing in 2015. The water samples were analyzed with a DNA-detection technique, called Polymerase Chain Reaction (PCR), which is a widely practiced molecular technique to detect trace amounts of very specific DNA sequences in water samples; this is more popularly thought of as “DNA fingerprinting”. Additionally, one to four locations on each stream have been periodically analyzed for pH, total dissolved solids, electrical conductivity, nitrates, and dissolved oxygen to look for chemical trends. Aerial views of the streams were obtained from Google Maps to determine the extent of their riparian buffers. As a check to this data, portions of each of the three streams were walked to document the riparian buffer zones along the streams. Also, a map with multiple layers was created in ArcMap that includes land use and sampling results. Definite correlation can be seen between precipitation and E. coli presence, therefore, samples were collected before, during, and immediately after precipitation events. Rainfall is a likely cause of spikes in E. coli results, due to run-off from these rainfall occurrences bypassing the natural filtration of the riparian buffer.
Geological Society of America Abstracts with Programs. Vol. 48, No. 7
doi: 10.1130/abs/2016AM-286685
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